Detection Testing For Cosmetics & Personal Care Products
Microbiological detection testing is a specialised branch of cosmetic testing focused on identifying and quantifying microbial contaminants present in cosmetic products.
Detection Testing
The presence of microorganisms in cosmetics and personal care products is a significant concern, as it can lead to various adverse effects.
Firstly, microorganisms can instigate product degradation, compromising its quality and efficacy over time. Moreover, microbial proliferation can result in spoilage, rendering the cosmetic product unfit for its intended purpose. Beyond product integrity concerns, the presence of microbial contaminants poses potential health risks to consumers.
Upon application or use of contaminated cosmetics and personal care products, individuals may experience adverse reactions ranging from mild skin irritations to more severe allergic reactions or infections. Particularly vulnerable populations, such as those with compromised immune systems, may face heightened risks from exposure to microbial contaminants in cosmetics.
Microbiological detection testing serves as a crucial safeguard against the proliferation of microbial contaminants in cosmetics and personal care products.
Our Methods
Below is a summary of our method for Detection Testing here at our UKAS accredited cosmetic testing laboratory Southern Microbiological Services:
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Homogenisation with Eugon Broth
A sample of 1ml or 1g of the cosmetic product is homogenized with 9ml of Eugon broth, creating a uniform suspension.
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Incubation
The suspension is then incubated at a precise temperature of 32.5±2.5°C for a minimum of 20 hours, allowing any potential microbial contaminants to proliferate.
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Streaking onto TSA Plate
Following the incubation period, a 10µl aliquot of the homogenised suspension is streaked onto a Tryptic Soy Agar (TSA) plate, ensuring even distribution.
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Incubation and Examination
The TSA plate is then incubated in an inverted position at 32.5±2.5°C for a duration of 48-72 hours, facilitating the growth of microbial colonies.
After the incubation period, plates are examined for microbial growth.
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Confirmation of Microbial Presence
All observed growth on the TSA plates is thoroughly examined and confirmed for the presence of 4 microbial contaminants, E. coli, P. aeruginosa, S. aureus, and C. albicans.
Microbiological detection testing, as outlined in our method above, serves as a critical defence against the proliferation of microbial contaminants within cosmetics and personal care products. By following a systematic approach that includes homogenisation, incubation, streaking onto TSA plates, and thorough examination, we are able to effectively identify and quantify potential microbial threats.
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